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Image Search Results
Journal: Frontiers in Physiology
Article Title: Health Effects of Alternate Day Fasting Versus Pair-Fed Caloric Restriction in Diet-Induced Obese C57Bl/6J Male Mice
doi: 10.3389/fphys.2021.641532
Figure Lengend Snippet: Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow cytometry. The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Article Snippet: Samples were analyzed using a Beckman Cytoflex flow cytometer and analyzed using Flowjo
Techniques: Staining, Flow Cytometry