cytoflex flow fluorescence cytometry Search Results


90
Becton Dickinson flow cytometry analysis software
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Flow Cytometry Analysis Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cytoflex flow cytometer
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beckman Coulter cytoflex lx flow cytometry system
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex Lx Flow Cytometry System, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beckman Coulter cytoflex flow cytometer
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex Flow Cytometer, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc cytoflex flow cytometers
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex Flow Cytometers, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc cytoflex s flow cytometer
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex S Flow Cytometer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc flow cytometry
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Flow Cytometry, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cytoflex lx
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex Lx, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson flow cytometry cytoflex-lx
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Flow Cytometry Cytoflex Lx, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Health Ltd cytoflex flow cytometer
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Cytoflex Flow Cytometer, supplied by Bio-Health Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10X Genomics research flow cytometers cytoflex software cellranger
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
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Miltenyi Biotec macs separator miltenyi 130 042 302 cytoflex s flow cytometer beckman n a facs aria ii bd biosciences n a
Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow <t>cytometry.</t> The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.
Macs Separator Miltenyi 130 042 302 Cytoflex S Flow Cytometer Beckman N A Facs Aria Ii Bd Biosciences N A, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow cytometry. The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.

Journal: Frontiers in Physiology

Article Title: Health Effects of Alternate Day Fasting Versus Pair-Fed Caloric Restriction in Diet-Induced Obese C57Bl/6J Male Mice

doi: 10.3389/fphys.2021.641532

Figure Lengend Snippet: Single cell suspensions from the spleen, blood, and mesenteric lymph nodes were stained with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and analyzed by flow cytometry. The lymphocyte population was gated based on FSC vs SSC profiles then CD3 + cells gated and used as the parent population for CD4 + and CD8 + T cell identification. Graphs show mean and SD of the percentage of CD4 + and CD8 + T cells of lymphocytes. (A) Frequency of T cells in the spleen. All diet regimens had a lower percentage of splenic CD4 + and CD8 + T cells compared to the CON group. The LF group had a significantly higher percentage of CD4 + and CD8 + T cells compared to the HF group. (B) Frequency of T cells in the blood. HF, ADF, and PF groups had reduced frequencies of CD4+ and CD8+ T cells in the blood, while the LF group showed no significant reduction. (C) Frequency of T cells in the mesenteric lymph node. HF, ADF, and PF groups had reduced frequencies of T cells in the mesenteric lymph nodes while the LF group showed no significant reductions in T cell frequency. a: p < 0.05 vs. CON; b: p < 0.05 vs. HF; c: p < 0.05 vs. LF for each respective lymphocyte population.

Article Snippet: Samples were analyzed using a Beckman Cytoflex flow cytometer and analyzed using Flowjo flow cytometry analysis software (FlowJo LLC, OR).

Techniques: Staining, Flow Cytometry